High-performance liquid chromatography is a suitable method to Separate, identify, and quantify each component in a mixture. HPLC method has many applications in different sciences because of its high sensitivity, fast adaptation for making correct quantitative measurements, and separation of non-volatile or volatile species against heat. In this method, there is a stationary phase and a mobile phase. The mixture of chemicals is carried through the stationary phase by the flow of a mobile phase. Separations are based on differences in transfer rates among sample components.
Some of the HPLC applications include: separation and quantitative determination of
- Fat soluble vitamins (A,D,E and K)
- Water soluble vitamins (B-complex vitamins such as B1, B2, B3, B6, Folic acid, Pantothenic acid, B12, VitaminC)
- Residual pesticides such as 2, 4-D and Monochrotophos.
- Antioxidants such as TBHQ, BHA and BHT.
- Sugars: Glucose, Fructose, Maltose and other saccharides.
- Cholesterol and sterols
- Dyes and synthetic colours.
- Mycotoxins such as Aflatoxins B1,B2,G1,G2,M1,M2and ochratoxin
- Amino acids
- Residual antibiotics
- Steroids and Flavonoids
- Aspartame and other artificial sweeteners.
- Active ingredients in farms such as alliin in garlic and catechin in tea extracts.