High-performance liquid chromatography is a suitable method to Separate, identify, and quantify each component in a mixture. HPLC method has many applications in different sciences because of its high sensitivity, fast adaptation for making correct quantitative measurements, and separation of non-volatile or volatile species against heat. In this method, there is a stationary phase and a mobile phase. The mixture of chemicals is carried through the stationary phase by the flow of a mobile phase. Separations are based on differences in transfer rates among sample components.

Some of the HPLC applications include: separation and quantitative determination of

  • Fat soluble vitamins (A,D,E and K)
  • Water soluble vitamins (B-complex vitamins such as B1, B2, B3, B6, Folic acid, Pantothenic acid, B12, VitaminC)
  • Residual pesticides such as 2, 4-D and Monochrotophos.
  • Proteins
  • Antioxidants such as TBHQ, BHA and BHT.
  • Sugars: Glucose, Fructose, Maltose and other saccharides.
  • Cholesterol and sterols
  • Dyes and synthetic colours.
  • Mycotoxins such as Aflatoxins B1,B2,G1,G2,M1,M2and ochratoxin
  • Amino acids
  • Residual antibiotics
  • Steroids and Flavonoids
  • Aspartame and other artificial sweeteners.
  • Active ingredients in farms such as alliin in garlic and catechin in tea extracts.
HPLC-  Chromatography